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Crude protein min. 38.0% Crude fat min. 18.0% Crude fiber max. 3.5% Moisture max. 10.0% Calcium min. 1.2% Phosphorus min. 1.0% Vitamin A min. 20,000 IU/kg Vitamin D3 min. 1,300 IU/kg Vitamin E min. 130 IU/kg Omega-3 fatty acids* min. 0.4% Omega-6 fatty acids* min. 3.5% Glucosamine* min. 1000 mg/kg Chondroitin sulfate* min. 600 mg/kg Total microorganisms* min. 225,000,000 CFU/lb (L. acidophilus, L. casei, B. bifidum, E. faecium in descending amounts) *Not recognized as an essential nutrient by the AAFCO Dog Food Nutrient Profiles. Calorie Content (calculated): 3,895 kcal/kg or 485 kcal/cup ME Nutrience Grain Free Subzero Prairie Red for large breed dogs is formulated to meet the nutritional levels established by the AAFCO Dog Food Nutrient Profiles for all life stages except for growth of large size puppies (70 lb. or more as an adult).